School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations. General Summary: A full-time research technician position is available at the Sipkins Lab in the Dept. of Medicine at Duke University. The lab seeks to define the molecular characteristics of tissue microenvironments that foster the survival and regeneration of both normal and cancerous stem cells. In combination with molecular and cell biological approaches, the lab utilizes state-of-the-art in vivo multiphoton and confocal optical imaging techniques to explore these questions. This position is ideal for students taking a gap years and applying to medical or graduate school. Essential Functions: 1 Assist in general laboratory maintenance and administration. This includes but is not limited to ordering, inventory, writing of standard operating procedures and supporting other lab personnel. Estimated effort: 25% 2 Mammalian tissue culture care of cell lines, primary cells, freezing cells, transfection, clonal isolation, histology, immunohistochemistry/immunofluorescence, flow cytometry, microscopy fluorescence, confocal, mouse work general care, breeding, minor surgical procedures, dissection, and molecular biology procedures RNA/DNA isolation, RT-PCR, subcloning. Estimated effort: 60% 3 Maintain accurate, up-to-date and well-documented records of procedures performed lab notebook, instrument logs, etc and method development. Estimated effort: 15% Qualifications: A bachelor’s degree in Biology, Chemistry, or related discipline, or the equivalent combination of education and training in which comparable skills have been acquired. Must have a general understanding of Molecular and Cell Biology concepts. Must have demonstrated basic laboratory research skills, including skills in making solutions and reagents. Strong familiarity with Word, Excel and PowerPoint desired. Must be open to working with mice. Excellent communication and organizational skills are pluses. Preferences: Attention to detail, strong oral and written communication skills,
